gel preparation Search Results


buffer components  (Chem Impex International)
95
Chem Impex International buffer components
Buffer Components, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
buffer components - by Bioz Stars, 2026-06
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cydye dige fluor cy 3 saturation dye  (GE Healthcare)
93
GE Healthcare cydye dige fluor cy 3 saturation dye
Mitochondria were untreated or incubated with MitoSNO, and labelled with Cy5 maleimide (red) following either S-nitrosothiol specific reduction ( A , <t>SNO-DIGE)</t> or non-specific reduction ( B , Redox-DIGE). Cy5-labelled samples were combined separately with a <t>Cy3-labelled</t> sample (green) pooled from all replicate experiments and resolved on the same 2D gel. The superimposed fluorescent scans of representative gels from each triplicate experimental condition are shown. Gels used for identification of proteins of interest by MS were subsequently stained with Coomassie Blue ( C ).
Cydye Dige Fluor Cy 3 Saturation Dye, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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sds page gel preparation kit  (Beyotime)
99
Beyotime sds page gel preparation kit
Mitochondria were untreated or incubated with MitoSNO, and labelled with Cy5 maleimide (red) following either S-nitrosothiol specific reduction ( A , <t>SNO-DIGE)</t> or non-specific reduction ( B , Redox-DIGE). Cy5-labelled samples were combined separately with a <t>Cy3-labelled</t> sample (green) pooled from all replicate experiments and resolved on the same 2D gel. The superimposed fluorescent scans of representative gels from each triplicate experimental condition are shown. Gels used for identification of proteins of interest by MS were subsequently stained with Coomassie Blue ( C ).
Sds Page Gel Preparation Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
sds page gel preparation kit - by Bioz Stars, 2026-06
99/100 stars
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carboxyphenol ba  (Chem Impex International)
95
Chem Impex International carboxyphenol ba
Mitochondria were untreated or incubated with MitoSNO, and labelled with Cy5 maleimide (red) following either S-nitrosothiol specific reduction ( A , <t>SNO-DIGE)</t> or non-specific reduction ( B , Redox-DIGE). Cy5-labelled samples were combined separately with a <t>Cy3-labelled</t> sample (green) pooled from all replicate experiments and resolved on the same 2D gel. The superimposed fluorescent scans of representative gels from each triplicate experimental condition are shown. Gels used for identification of proteins of interest by MS were subsequently stained with Coomassie Blue ( C ).
Carboxyphenol Ba, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
carboxyphenol ba - by Bioz Stars, 2026-06
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sds page gel preparation kit  (Boster Bio)
98
Boster Bio sds page gel preparation kit
Mitochondria were untreated or incubated with MitoSNO, and labelled with Cy5 maleimide (red) following either S-nitrosothiol specific reduction ( A , <t>SNO-DIGE)</t> or non-specific reduction ( B , Redox-DIGE). Cy5-labelled samples were combined separately with a <t>Cy3-labelled</t> sample (green) pooled from all replicate experiments and resolved on the same 2D gel. The superimposed fluorescent scans of representative gels from each triplicate experimental condition are shown. Gels used for identification of proteins of interest by MS were subsequently stained with Coomassie Blue ( C ).
Sds Page Gel Preparation Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 1 article reviews
sds page gel preparation kit - by Bioz Stars, 2026-06
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page gel fast preparation kit epizyme  (Epizyme Inc)
86
Epizyme Inc page gel fast preparation kit epizyme
Mitochondria were untreated or incubated with MitoSNO, and labelled with Cy5 maleimide (red) following either S-nitrosothiol specific reduction ( A , <t>SNO-DIGE)</t> or non-specific reduction ( B , Redox-DIGE). Cy5-labelled samples were combined separately with a <t>Cy3-labelled</t> sample (green) pooled from all replicate experiments and resolved on the same 2D gel. The superimposed fluorescent scans of representative gels from each triplicate experimental condition are shown. Gels used for identification of proteins of interest by MS were subsequently stained with Coomassie Blue ( C ).
Page Gel Fast Preparation Kit Epizyme, supplied by Epizyme Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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10% sds-page gels p1200  (Beijing Solarbio Science)
90
Beijing Solarbio Science 10% sds-page gels p1200
Mitochondria were untreated or incubated with MitoSNO, and labelled with Cy5 maleimide (red) following either S-nitrosothiol specific reduction ( A , <t>SNO-DIGE)</t> or non-specific reduction ( B , Redox-DIGE). Cy5-labelled samples were combined separately with a <t>Cy3-labelled</t> sample (green) pooled from all replicate experiments and resolved on the same 2D gel. The superimposed fluorescent scans of representative gels from each triplicate experimental condition are shown. Gels used for identification of proteins of interest by MS were subsequently stained with Coomassie Blue ( C ).
10% Sds Page Gels P1200, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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sdspage gel  (Beijing Solarbio Science)
90
Beijing Solarbio Science sdspage gel
Mitochondria were untreated or incubated with MitoSNO, and labelled with Cy5 maleimide (red) following either S-nitrosothiol specific reduction ( A , <t>SNO-DIGE)</t> or non-specific reduction ( B , Redox-DIGE). Cy5-labelled samples were combined separately with a <t>Cy3-labelled</t> sample (green) pooled from all replicate experiments and resolved on the same 2D gel. The superimposed fluorescent scans of representative gels from each triplicate experimental condition are shown. Gels used for identification of proteins of interest by MS were subsequently stained with Coomassie Blue ( C ).
Sdspage Gel, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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in-gel digestion and sample preparation protocol  (Protea Bio)
90
Protea Bio in-gel digestion and sample preparation protocol
Mitochondria were untreated or incubated with MitoSNO, and labelled with Cy5 maleimide (red) following either S-nitrosothiol specific reduction ( A , <t>SNO-DIGE)</t> or non-specific reduction ( B , Redox-DIGE). Cy5-labelled samples were combined separately with a <t>Cy3-labelled</t> sample (green) pooled from all replicate experiments and resolved on the same 2D gel. The superimposed fluorescent scans of representative gels from each triplicate experimental condition are shown. Gels used for identification of proteins of interest by MS were subsequently stained with Coomassie Blue ( C ).
In Gel Digestion And Sample Preparation Protocol, supplied by Protea Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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skin preparation gel skinpure  (Nihon Kohden corporation)
90
Nihon Kohden corporation skin preparation gel skinpure
Mitochondria were untreated or incubated with MitoSNO, and labelled with Cy5 maleimide (red) following either S-nitrosothiol specific reduction ( A , <t>SNO-DIGE)</t> or non-specific reduction ( B , Redox-DIGE). Cy5-labelled samples were combined separately with a <t>Cy3-labelled</t> sample (green) pooled from all replicate experiments and resolved on the same 2D gel. The superimposed fluorescent scans of representative gels from each triplicate experimental condition are shown. Gels used for identification of proteins of interest by MS were subsequently stained with Coomassie Blue ( C ).
Skin Preparation Gel Skinpure, supplied by Nihon Kohden corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
skin preparation gel skinpure - by Bioz Stars, 2026-06
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gelase agarose gel-digesting preparation  (Epicentre Biotechnologies)
90
Epicentre Biotechnologies gelase agarose gel-digesting preparation
Mitochondria were untreated or incubated with MitoSNO, and labelled with Cy5 maleimide (red) following either S-nitrosothiol specific reduction ( A , <t>SNO-DIGE)</t> or non-specific reduction ( B , Redox-DIGE). Cy5-labelled samples were combined separately with a <t>Cy3-labelled</t> sample (green) pooled from all replicate experiments and resolved on the same 2D gel. The superimposed fluorescent scans of representative gels from each triplicate experimental condition are shown. Gels used for identification of proteins of interest by MS were subsequently stained with Coomassie Blue ( C ).
Gelase Agarose Gel Digesting Preparation, supplied by Epicentre Biotechnologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
gelase agarose gel-digesting preparation - by Bioz Stars, 2026-06
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dc alufolien silica gel 60 f 254 (0.5)  (Merck KGaA)
90
Merck KGaA dc alufolien silica gel 60 f 254 (0.5)
Mitochondria were untreated or incubated with MitoSNO, and labelled with Cy5 maleimide (red) following either S-nitrosothiol specific reduction ( A , <t>SNO-DIGE)</t> or non-specific reduction ( B , Redox-DIGE). Cy5-labelled samples were combined separately with a <t>Cy3-labelled</t> sample (green) pooled from all replicate experiments and resolved on the same 2D gel. The superimposed fluorescent scans of representative gels from each triplicate experimental condition are shown. Gels used for identification of proteins of interest by MS were subsequently stained with Coomassie Blue ( C ).
Dc Alufolien Silica Gel 60 F 254 (0.5), supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Image Search Results


Mitochondria were untreated or incubated with MitoSNO, and labelled with Cy5 maleimide (red) following either S-nitrosothiol specific reduction ( A , SNO-DIGE) or non-specific reduction ( B , Redox-DIGE). Cy5-labelled samples were combined separately with a Cy3-labelled sample (green) pooled from all replicate experiments and resolved on the same 2D gel. The superimposed fluorescent scans of representative gels from each triplicate experimental condition are shown. Gels used for identification of proteins of interest by MS were subsequently stained with Coomassie Blue ( C ).

Journal: Biochemical Journal

Article Title: Identification of S-nitrosated mitochondrial proteins by S -nitrosothiol difference in gel electrophoresis (SNO-DIGE): implications for the regulation of mitochondrial function by reversible S-nitrosation

doi: 10.1042/BJ20100633

Figure Lengend Snippet: Mitochondria were untreated or incubated with MitoSNO, and labelled with Cy5 maleimide (red) following either S-nitrosothiol specific reduction ( A , SNO-DIGE) or non-specific reduction ( B , Redox-DIGE). Cy5-labelled samples were combined separately with a Cy3-labelled sample (green) pooled from all replicate experiments and resolved on the same 2D gel. The superimposed fluorescent scans of representative gels from each triplicate experimental condition are shown. Gels used for identification of proteins of interest by MS were subsequently stained with Coomassie Blue ( C ).

Article Snippet: Specific reduction of S -nitrosothiols in the samples was achieved by addition of 1 mM ascorbate and 10 μM CuSO 4 in Chelex 100-treated PBS buffer in the presence of 40 μM CyDye™ DIGE Fluor Cy™3 saturation dye (Cy3) or CyDye™ DIGE Fluor Cy™5 saturation dye [Cy5 (indodicarbocyanine)] (GE Healthcare).

Techniques: Incubation, Two-Dimensional Gel Electrophoresis, Staining